Determination of vanilmandelic acid and homovanillic acid in urine by gas chromatography.
نویسندگان
چکیده
منابع مشابه
Determination of urinary vanilmandelic acid and homovanillic acid by high performance liquid chromatography with amperometric detection.
A method was developed for the simultaneous determination of urinary vanilmandelic acid and homovanillic acid, which included a two step prepurification and a reversed-phase high-performance liquid chromatography with amperometric detection. Conditions were evaluated for performing measurements with the amperometric detector free from electric interference. The method was linear between 2.5 and...
متن کاملDEVELOPMENT OF A METHOD FOR THE DETERMINATION OF 4-HYDROXY-3-METHOXYMANDELIC ACID (VANILLYLMANDELIC ACID) IN URINE BY PAPER CHROMATOGRAPHY
We have developed a simple and precise paper chromatographic method for the determination of 4-hydroxy-3-methoxymandelic acid (VMA) in urine. Concentrations of VMA in patients with neuroblastoma were increased in comparison to controls. The linearity was excellent in the concentration range tested. The within-assay coefficient of variation for control and patient urine was less than 2.2%. ...
متن کاملThe determination of oxalic acid in plasma and urine by means of capillary gas chromatography.
A gas chromatographic procedure for determining oxalate in plasma is described, in which the trimethylsilyl derivative of oxalate is analyzed on a 25-m capillary SE-30 column. In addition the effects of standing of whole blood or plasma and the effect of added glyoxalate on the oxalate concentration were studied. The present method offers good specificity and sensitivity and is easy to perform,...
متن کاملElectrophoretic determination of vanilmandelic acid (VMA) in urine by direct application to cellulose acetate.
‘THE VALUE of vanilmandelic acid (3-methoxy-4-hydroxymandelic acid, 1/MA) in urine for the diagnosis of pheochromocytoma is well recognized. The comprehensive review of VMA analysis by Sunderman (1) reiterates that there is a large difference between normal and pathologic 24-hr. urinary excretion of 1/MA. Such a sharp difference in concentration makes VMA analysis adaptable to a semiquantitativ...
متن کاملDetermination of mandelic acid enantiomers in urine by derivatization in supercritical carbon dioxide prior to their determination by gas chromatography.
The use of a supercritical carbon dioxide reaction medium for the determination of the (R)- and (S)-enantiomers of mandelic acid (MA) is proposed. The process involves a previous derivatization step under supercritical conditions by which the carboxyl group is esterified with methanol, then followed by acylation of the hydroxyl group in methyl MA with pentafluoropropionic anhydride in the absen...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Chemical and Pharmaceutical Bulletin
سال: 1975
ISSN: 0009-2363,1347-5223
DOI: 10.1248/cpb.23.3040